Vitamin D guide

How to use vitamin D

6 questions to ask when reading the label and your test result.

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Six questions before the numbers on the label.

Vitamin D labels put units such as IU, µg and %NRV side by side. A lab report shows ng/mL or nmol/L. This guide goes through the six questions to ask when reading the label and your test result, in order. Under each question you will find what to look for and how that information appears on Uth-FastD™. The guide does not rank products and does not suggest a personal amount.

  1. Are vitamin D and D3 the same?
  2. IU, µg, units: how do you convert?
  3. What does %NRV on the label mean?
  4. How much vitamin D a day?
  5. When and how do you take it?
  6. How do you read a test result?

Are vitamin D and D3 the same?

Vitamin D is the name of a group. Supplements most often use D3 (cholecalciferol). D2 (ergocalciferol) and calcidiol (calcifediol, 25(OH)D3) are also forms in this group.

D3 is the form made in the skin with the UVB in sunlight, and the form found in animal foods. In supplements it usually comes from sheep's wool fat (lanolin), and in vegan products from lichen. D2 comes from plants and fungi. The D3 that enters the body is first converted in the liver into 25(OH)D3, that is, calcidiol (calcifediol). This is the form measured in a blood test. Calcidiol is the form in which the liver conversion step is already complete. It is then converted into the active form in the kidneys. We explain this path step by step on the science of vitamin D page.

How do you find the form on the label?

  1. Look for the form in the ingredients list. It is often written in brackets, for example “Vitamin D3 (Cholecalciferol)” or “Vitamin D3 (Calcidiol Monohydrate)”. For D2, the word “ergocalciferol” is used.
  2. The “Vitamin D3” row in the nutrition table shows only the amount. The form is written in the ingredients list. If the list does not name the form, you can ask the manufacturer.
  3. If you are looking for a vegetarian or vegan product, check the suitability statement on the pack. D3 can come from animal (lanolin) or plant-based (lichen, yeast) sources.

IU, µg, units: how do you convert?

1 µg (microgram) of vitamin D is 40 IU. To go from IU to µg, divide by 40. To go from µg to IU, multiply by 40.

Because vitamin D is used in very small amounts, you will see two units on labels. µg (microgram, sometimes written “mcg”) is a unit of weight, one millionth of a gram. IU (International Unit) is a unit defined by the biological activity of the vitamin. For vitamin D, the relationship between the two is fixed: 1 µg is 40 IU.[6] In Türkiye, supplement labels give the amount in µg, often with the IU value next to it. People often search for “vitamin D 1000 mg”, but vitamin D is not measured in milligrams. 1000 IU is 25 µg, or 0.025 mg.

Calculator: IU and µg

400 IU10 µg
600 IU15 µg
800 IU20 µg
1000 IU25 µg
2000 IU50 µg
4000 IU100 µg
50,000 IU1250 µg

How do you read the amount on the label?

  1. Check whether the amount is given in µg or IU.
  2. Divide the IU value by 40 to convert it to µg: 2000 IU ÷ 40 = 50 µg.
  3. For drops, the amount may be given per 1 drop or per 1 ml. If the label says 400 IU per drop, that is 10 µg. To find the daily amount, multiply the amount per drop by the number of drops you use.

What does %NRV on the label mean?

NRV is the nutrient reference value, and for vitamin D it is 5 µg. The “%NRV” on the label shows the amount in one serving as a share of this value.

On supplement labels, a “%NRV” column sits next to the nutrition table. Under the Turkish Food Codex, the nutrient reference value for vitamin D is 5 µg (200 IU).[3] This is a labelling measure, not an amount recommended for everyone. That is why %NRV can look high: a serving with 25 µg (1000 IU) equals 500% of the NRV. The percentage is calculated only against the reference used on the label and does not show your personal need. Personal need varies with age, time spent in the sun and diet.

How do you calculate %NRV?

  1. Divide the µg value in one serving by 5 and multiply by 100: 25 µg ÷ 5 × 100 = 500%.
  2. Check how many tablets, drops or ml make up a serving. %NRV is always given per serving.
  3. %NRV lets you read two labels on the same scale. It does not show a personal target.

How much vitamin D a day?

For a supplement, the measure is the recommended daily serving on the label. According to EFSA, the adequate intake for adults is 15 µg (600 IU) and the safe upper limit is 100 µg (4000 IU).

EFSA has set the adequate daily intake for adults and children over 1 year at 15 µg (600 IU). This value was calculated for conditions in which the skin makes little vitamin D.[1] The highest amount not expected to pose a health risk when taken every day over a long period, the upper limit, is 100 µg (4000 IU) a day for adults and 11–17-year-olds, and 50 µg (2000 IU) for 1–10-year-olds.[2] The upper limit includes the total from supplements, fortified foods and other foods. High doses such as 50,000 IU (1250 µg) are used as medicines, under a physician's supervision. You can review your own needs with your physician, based on your blood test result.

How do you work out your daily total?

  1. Find the recommended daily serving on the label and do not exceed it.
  2. If you use more than one supplement (a multivitamin, drops, combined products), add up the vitamin D in each of them in µg.
  3. Consult your physician or pharmacist before using it together with another vitamin D supplement.

When and how do you take it?

A particular time of day is not required. Products containing cholecalciferol (D3) are best taken after eating, with a meal that contains fat. Products containing calcidiol have no such requirement.

“Should vitamin D be taken in the morning or the evening?” is a common question. There is no strong evidence that the time of day changes the result. D3 is a fat-soluble vitamin. That is why supplements containing D3 are usually taken with a meal that contains fat. In one study, the same amount of D3 was absorbed in greater amounts when taken with a meal containing fat than with a fat-free meal.[4] Calcidiol, on the other hand, is a more polar molecule than D3, which means it dissolves more easily in water, and its absorption from the gut depends less on fat and bile.[10] So products containing calcidiol do not need to be taken with a fatty meal. A practical approach is to pair the supplement with the same meal every day. That makes it harder to forget. When choosing between drops, tablets, capsules and sprays, you can look at how easy the dose is to measure and which one you find easier. The dose for each is written on the label.

How do you read the instructions for use?

  1. Find the form in the ingredients list. If it is cholecalciferol (D3), take the product with a meal that contains fat. Products containing calcidiol have no such requirement.
  2. Check whether the label gives an instruction such as “with food” or “on an empty or full stomach”.
  3. For drops, check whether the dose is given in drops or ml.
  4. Pair the supplement with the same meal every day.

How do you read a test result?

In a blood test, the vitamin D level is measured as 25(OH)D. The result is given in ng/mL or nmol/L. Multiply the ng/mL value by 2.5 to get nmol/L.

On a lab report, this value appears under names such as “25-OH vitamin D”, “25(OH)D” or “vitamin D (25-hydroxy)”. Laboratories give the result in one of two units: 20 ng/mL corresponds to 50 nmol/L. “What should my vitamin D level be?” has no single answer for everyone. In its 2011 report, the US Institute of Medicine (IOM) took 20 ng/mL (50 nmol/L) as the level that meets the needs of the large majority of the population.[5] The Endocrine Society's 2024 guideline does not recommend routine testing in healthy people and does not set a specific target value.[7] So review your result against the reference range on your report, together with your physician. You can find measurement data from Türkiye on the science of vitamin D page.

Calculator: ng/mL and nmol/L

10 ng/mL25 nmol/L
20 ng/mL50 nmol/L
30 ng/mL75 nmol/L
50 ng/mL125 nmol/L

The factor is approximate (the exact value is 2.496). The calculator does not interpret the result. It only converts the unit.

How do you read your report?

  1. Look for 25(OH)D or 25-hydroxy in the name of the test.
  2. Check the unit: multiply the ng/mL value by 2.5 to convert it to nmol/L, or divide the nmol/L value by 2.5 to convert it to ng/mL.
  3. Compare the result with the reference range on your report and review it with your physician.
A short addition

Where is vitamin D found?

The main source of vitamin D is the sun. The skin makes D3 with the UVB in sunlight. The amount it makes depends on the season, the time of day, your latitude, skin colour, age and sunscreen use.[6] In food, vitamin D is found in only a few sources. 85 g of cooked salmon contains about 14.2 µg (570 IU) of vitamin D, and one large egg about 1.1 µg (44 IU).[6] Fruit and vegetables contain almost no vitamin D. Food supplements are not a substitute for a varied diet.

Foods that contain vitamin D

  • Oily fish such as salmon, mackerel and sardines
  • Egg yolk
  • Mushrooms exposed to UV light
  • Foods with added vitamin D (fortified foods)

What does vitamin D do? Authorised claims*

  • Vitamin D contributes to normal absorption/utilisation of calcium and phosphorus.*
  • Vitamin D contributes to normal blood calcium levels.*
  • Vitamin D contributes to the maintenance of normal bones.*
  • Vitamin D contributes to the maintenance of normal muscle function.*
  • Vitamin D contributes to the maintenance of normal teeth.*
  • Vitamin D contributes to the normal function of the immune system.*
  • Vitamin D has a role in the process of cell division.*

Source: EFSA NDA Panel[8] and the Turkish Food Codex Regulation on Nutrition and Health Claims[9]. The claims belong to the nutrient (vitamin D), not to the product.

For the box in your hand

Label checklist.

This list is a short version of the six questions above. Look at the box in your hand and your lab report, and tick each item you can find the answer to. You can ask the manufacturer or your pharmacist for anything you cannot find. The list does not score a product. It only checks whether the information is on the label or in the report.

This list does not recommend a product and is not a health assessment. Your ticks are not saved and are cleared when you close the page.

Short test · Uth-FastD™

Vitamin D test. A short look at your lifestyle.

Look at your sun, eating and supplement routine in a few questions. This is not a health assessment and does not diagnose anything. Leave your email address at the end of the test: when Uth-FastD™ goes on sale, your 20% launch code is sent to your email. The test is currently in Turkish only.

Take the Vitamin D TestStart the Test

Frequently asked questions.

D3 is one form of vitamin D. Vitamin D is the name of a group that covers D2, D3 and the forms the body makes from them. Supplements most often use D3 (cholecalciferol). The form measured in a blood test is 25(OH)D, that is, calcidiol.

1000 IU is 25 µg (mcg). For vitamin D, 1 µg equals 40 IU. To convert IU to µg, divide by 40. To convert µg to IU, multiply by 40. For example, 2000 IU is 50 µg and 400 IU is 10 µg.

There is no strong evidence that a particular time of day changes the result. What matters is the form. Cholecalciferol (D3) dissolves in fat, so products containing D3 are best taken after eating, with a meal that contains fat. Calcidiol dissolves more easily in water and its absorption depends less on fat (Quesada-Gomez and Bouillon, 2018), so products containing calcidiol do not need a fatty meal. Follow the instructions on the label and take the supplement in the same routine every day.

There is no single target value for everyone. A blood test measures 25(OH)D and gives the result in ng/mL or nmol/L (ng/mL × 2.5 = nmol/L). The IOM's 2011 report takes 20 ng/mL (50 nmol/L) as its reference. The Endocrine Society's 2024 guideline does not set a specific target value. Review your result against the reference range on your report and with your physician.

Few foods contain vitamin D: oily fish such as salmon, mackerel and sardines, egg yolk, and mushrooms exposed to UV light. Fruit and vegetables contain almost none. The main source is D3 made in the skin with the UVB in sunlight.

For both, what matters is the amount on the label and regular use. With drops, the dose is given in drops or ml, and you need to read the amount per drop on the label. With tablets, the dose is fixed. A practical approach is to use whichever you find easier, and use it regularly.

Calcidiol (calcifediol, 25(OH)D3) is the form D3 is converted into in the liver. It is the form measured as your vitamin D level in a blood test. This form, in which the liver conversion step is already complete, is also used in supplements as calcidiol monohydrate.

What comes next?

The six questions help you read a vitamin D label and a lab report. You can find the path of vitamin D in the body, and where calcidiol sits on that path, on the science page. The small tablet that contains calcidiol is on the Uth-FastD™ page.

The science of vitamin D

D3, calcidiol and the active form: why a blood test looks at 25(OH)D, and measurement data from Türkiye.

Science page

Uth-FastD™

A small, easy-to-swallow tablet containing calcidiol monohydrate. One tablet a day, for ages 11 and over.

Meet the product

How to choose an omega-3

Six criteria to check on an omega-3 box and test report: amount, form, purity, independent testing, heavy metals and freshness.

Read the guide

The sources we cite on this page.

The sources reflect official scientific assessments of vitamin D, regulatory texts and the general scientific literature. They do not carry any product-specific clinical outcome claim.

  1. EFSA NDA Panel. Dietary reference values for vitamin D. EFSA Journal 2016;14(10):e04547. https://doi.org/10.2903/j.efsa.2016.4547
  2. EFSA NDA Panel. Scientific opinion on the tolerable upper intake level for vitamin D, including the derivation of a conversion factor for calcidiol monohydrate. EFSA Journal 2023;21(8):8145. https://doi.org/10.2903/j.efsa.2023.8145
  3. Turkish Food Codex Regulation on Food Labelling and Consumer Information (Official Gazette, 26.01.2017, no. 29960), nutrient reference values for vitamins and minerals. https://www.resmigazete.gov.tr/ (in Turkish)
  4. Dawson-Hughes B, Harris SS, Lichtenstein AH, Dolnikowski G, Palermo NJ, Rasmussen H. Dietary fat increases vitamin D-3 absorption. J Acad Nutr Diet 2015;115(2):225-30. https://doi.org/10.1016/j.jand.2014.09.014
  5. Ross AC, Manson JE, Abrams SA, et al. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know. J Clin Endocrinol Metab 2011;96(1):53-8. https://doi.org/10.1210/jc.2010-2704
  6. NIH Office of Dietary Supplements. Vitamin D: Fact Sheet for Health Professionals (unit conversion, food sources, factors that affect production in the skin). https://ods.od.nih.gov/factsheets/VitaminD-HealthProfessional/
  7. Demay MB, Pittas AG, Bikle DD, et al. Vitamin D for the prevention of disease: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 2024;109(8):1907-47. https://doi.org/10.1210/clinem/dgae290
  8. EFSA NDA Panel. Scientific Opinion on the substantiation of health claims related to vitamin D. EFSA Journal 2009;7(9):1227. https://doi.org/10.2903/j.efsa.2009.1227
  9. Turkish Food Codex Regulation on Nutrition and Health Claims and its annexed List of Health Claims (Official Gazette). https://www.resmigazete.gov.tr/ (in Turkish)
  10. Quesada-Gomez JM, Bouillon R. Is calcifediol better than cholecalciferol for vitamin D supplementation? Osteoporos Int 2018;29(8):1697-711. https://doi.org/10.1007/s00198-018-4520-y

If you would like to read more widely, our other guides are on the Longevity Insights page.

*Health claims apply when the nutrient is consumed in sufficient amounts. This page is for information only. It is not a product recommendation or a health assessment. Food supplements are not medicines, and food supplements are not a substitute for a varied diet. If you are pregnant or breastfeeding, take medication or have a chronic condition, consult your physician. Do not exceed the recommended daily dose.